Photosynthetic CO2 Fixation at Air Levels of CO2 by Isolated Spinach Chloroplasts
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چکیده
منابع مشابه
The enhancement of CO2 fixation in isolated chloroplasts by low sulfite concentrations and by ascorbate.
The enhancement of CO2 fixation in isolated, intact spinach chloroplasts by ascorbate or by low sulfite concentrations (less than 1 mM) is strongly reduced or even abolished by the addition of superoxide dismutase (SOD). By the use of 35SO3(2-) it is demonstrated that the rate of sulfate formation is much lower than the sulfite induced increase in CO2 fixation. This indicates that the superoxid...
متن کاملCauses for the Disappearance of Photosynthetic CO(2) Fixation with Isolated Spinach Chloroplasts.
When isolated spinach chloroplasts are illuminated, photosynthesis and CO(2) fixation die off within 30 to 90 minutes. Even when air levels of CO(2) are used which maintain high and rate-saturating amounts of ribulose 1,5-bisphosphate inside the plastids, CO(2) fixation declines. The decline begins with a drop in activity of the ribulose 1,5-bishosphate carboxylase/oxygenase, specifically loss ...
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Reduced osmotic potentials inhibited the rate of CO(2) fixation by isolated intact spinach (Spinacia oleracea) chloroplasts. This inhibition was observed immediately after transfer of chloroplasts from a solution containing 0.33 m sorbitol to higher sorbitol concentrations, and the depressed rate remained constant. The inhibited CO(2) fixation could not be attributed to a decreased rate of phot...
متن کاملLevel of photosynthetic intermediates in isolated spinach chloroplasts.
The level of intermediates of the photosynthetic carbon cycle was measured in intact spinach chloroplasts in an attempt to determine the cause of the induction lag in CO(2) assimilation. In addition, transient changes in the level of the intermediates were determined as affected by a light-dark period and by the addition of an excess amount of bicarbonate during a period of steady photosynthesi...
متن کاملOxidative photosynthetic phosphorylation by spinach chloroplasts.
In an earlier note, evidence was presented for the occurrence of a new type of chloroplast-catalyzed photosynthetic phosphorylation called “oxidative photophosphorylation” (I). When spinach chloroplasts are illuminated in the presence of orthophosphate, adenosine diphosphate, and a dye such as trichlorophenol indophenol, adenosine triphosphate is generated provided oxygen is available, but not ...
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ژورنال
عنوان ژورنال: Plant Physiology
سال: 1982
ISSN: 0032-0889,1532-2548
DOI: 10.1104/pp.69.6.1263